Journal: PLoS ONE

Article Title: Reduced Level of the BCL11B Protein Is Associated with Adult T-Cell Leukemia/Lymphoma

doi: 10.1371/journal.pone.0055147

Figure Lengend Snippet: (A) Immunoblot analysis of BCL11B protein expression in a panel of HTLV-1-positive T-cell lines and HTLV-I-free T-cell lines. The cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with polyclonal antibodies against BCL11B (upper panel). Jurkat, MOLT4, PEER and SupT-11 are HTLV-I-free T -lymphoid leukemia cell lines; MT-2 and MT-4 are human T-cell lines transformed in vitro using HTLV-I; MT-1, Hut102, ATN-1, KKI, KOB, OMT RST and SO4 are ATLL patient-derived cell lines. The data were normalized to ß-tubulin (ß-TUB) and calibrated to the BCL11B/ß-tubulin ratio in the healthy volunteer samples as a relative expression value of 100 (lower panel). (B) RT-PCR analysis of BCL11B, Tax and FOXP3 mRNA in CD4+ T lymphocytes from healthy volunteers, HTLV-I-positive T-cell lines and HTLV-I-free T-cell lines (upper panel). (C) RT-qPCR analysis of BCL11B mRNA. The data were normalized to 28S ribosome RNA ( rRNA ) and calibrated to the BCL11B/28S -ribosome RNA ratio in the healthy volunteer sample as a relative expression value of 100 (lower panel). The data represent the mean values of the assays, which were performed in duplicate.

Article Snippet: The anti-human CD4 FITC-conjugated monoclonal and the goat anti-rabbit IgG DyLight 549-conjugated secondary antibodies were purchased from Abcam (Cambridge, UK).

Techniques: Western Blot, Expressing, SDS Page, Transformation Assay, In Vitro, Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR